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. 2020 Apr 5;21(7):2525. doi: 10.3390/ijms21072525

Figure 1.

Figure 1

Muscle gene expression and MyomiR profile in Zeb2-null and R26_Zeb2 mESCs subjected to myogenic differentiation. (A) Schematic representation of myogenic induction for mESCs. (B) The qRT-PCR analysis confirms the successful MyoD transient transfection in CTR, Zeb2-null and R26_Zeb2 mESCs. At 24, 48, and 72 h from transfection, data are represented as ΔCt, normalized for the housekeeping Gapdh. Values are shown as mean ± s.d.; n = 3, *** p < 0.0001. (C,D) qRT-PCR analysis for skeletal muscle genes (Pax3, Pax7, MyoD, Myogenin) in CTR, Zeb2-null and R26_Zeb2 mESCs, on day 22 from myogenic induction (C) or upon MyoD transfection (D). Each data point is represented as ΔCt, normalized for the housekeeping Gapdh. (E,F) qRT-PCR analysis for MyomiRs (miR-1, miR-133b, miR-208 and miR-206) in CTR, Zeb2-null and R26_Zeb2 mESCs, on day 22 from myogenic induction (E) or upon MyoD transfection (D). Each data point is represented as ΔCt, normalized for the housekeeping miR-16. Values are shown as mean ± SD; n = 3, * p < 0.05, ** p < 0.005, *** p < 0.0001.