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Smad7+ cells in muscles injected with Zeb2-null and R26_Zeb2 mESC derivatives. Immunofluorescence analysis of Smad7 (red), GFP (green), and laminin (magenta) in sham-operated ((A) and inset (A’)) muscles, or injected with CTR (Zeb2 flox/flox as control) ((B) and inset (B’)), Zeb2- null ((C) and inset (C’)), and R26_Zeb2 ((D) and inset (D’)) GFP+ mESC derivatives. Arrows indicate Smad7+. Nuclei were stained in blue with Hoechst; scale bars = 200 μm.
