Figure 4.

Effect of glucose concentration on MCF7 cell surface PI phosphorylation. PI3P, PI(3,4)P2, and PIP3 relative concentrations for MCF7s as determined by fluorescence intensity in fixed cells (n > 60). The % of total PI lipid for each mono-, di-, and tri-phosphorylated PI was determined through intensity analysis for the MCF7 cell line, incubated at 5.5 (☐, blue), 10 (☐, orange), 25 (☐, grey), and 50 (☐, yellow) mM serum glucose concentrations, respectively. * denotes p < 0.05 and ** denotes p < 0.005 as determined by a one-way ANOVA pair-wise comparison.