Figure 1.

Characterization of Tallyho (TH) mice. (A) Oral glucose tolerance test (OGTT). Wild-type mice (Wt, n_mice = 7); TH mice (TH, n_mice = 7). (B) Coronary flow velocity reserve (CFVR). Wt, n_mice = 6; TH, n_mice = 5. (C) Representative photographs showing the capillary density and EC apoptosis. ECs were stained by BS-lectin-FITC (a marker of ECs) and apoptotic cells were detected by TUNEL staining (red). An arrow indicates co-stained cells (apoptotic ECs, orange). Bar = 50µm. (D) Averaged data showing the capillary density in Wt (n_mice = 5) and TH (n_mice = 5). (E) Summarized data of the percentage of apoptotic ECs (the number of apoptotic ECs divided by total number of ECs). N_mice = 5 for each group. (F) Representative images of hearts after I/R. The area at risk (AAR) is shown in non-blue (= white + red) and the infarcted area is shown in white. Five cross sections (each 1 mm thick) from the ligation site to the apex. Averaged data of infarcted area in the AAR (G) and the AAR in the ventricle (H). N_mice = 6 per group. (I) Protein O-GlcNAcylation in MCECs was detected by the anti-O-GlcNAc antibody (RL2). Arrow indicates a representative band of proteins showing increased O-GlcNAcylation. Actin was used as a loading control. (J) Summarized data of the intensity of the band indicated by the arrow (left) and total RL2 signals (right) (n_mice = 7 per group). Data are mean ± S.E. *P < 0.05 vs. Wt. Statistical comparison between time-response curves was made by two-way ANOVA with Bonferroni post hoc test. Unpaired Student’s t-test was used for comparisons of two experimental groups.