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. 2020 Mar 31;21(7):2393. doi: 10.3390/ijms21072393

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of Calebin A and/or 5-FU on TNF-β-promoted colonosphere formation and migration in CRC cells in 3D-alginate tumor cultures. Serum-starved cultures of HCT116 (A,B,D,E) and HCT116R (C,F) cell lines in alginate matrix (stars) were treated as described in the Materials and Methods section. Colonosphere formation and migration were evaluated by light microscopy after 10 days. All experiments were performed at least three times. The number of colonospheres (arrows) was quantified by counting 100 cells from 20 different microscopic fields, and the number of invaded spheroids was quantified in each well. p < 0.05 (*) and p < 0.01 (**) indicate a significant difference compared to the control group. Magnification A: ×24, scale bar = 0.2 mm.