Figure 5.

Effect of Calebin A and/or 5-FU on TNF-β-promoted p65-NFκB activation and NF-κB-regulated gene end-products involved in apoptosis, proliferation and metastasis promoted by TNF-β in CRC cells Immunoblotting of whole-cell lysates from HCT116 (A) and HCT116R (B) in monolayer cultures treated as described in the Materials and Methods section was performed for anti-p65-NF-κB, anti-phospho-p65-NF-κB, anti-β1-integrin, anti-MMP-9, anti-CXCR4, anti-Ki67 and anti-cleaved-caspase-3. β-actin served as an internal loading control in all experiments. For densitometric evaluation, results are compared to control. p < 0.05 (*) and p < 0.01 (**) were considered statistically significant.