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Fluorimetric analysis of calpain activity in platelets that were incubated for 180 min with a mixture of 10 μg/mL PF4 + 50 μg/mL KKO or RTO. The numbers that characterize the calpain activity were normalized by the corresponding negative control (untreated platelets) taken as 100%. Experiments were performed with platelets isolated from seven independent donors. * p < 0.05 (Mann–Whitney U test).
