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. 2020 Apr 3;21(7):2481. doi: 10.3390/ijms21072481

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Characterization of the iPSC-derived MΦ. (A) Morphology of CD34iPSC16exi-iMΦ and iCasp9—iMΦ on May-Grünwald-Giemsa-stained cytospins (scale bar—50 µm). (B) Flow cytometric detection of hematopoietic (CD45) and MΦ-specific (CD14, CD163, CD11b) surface marker expression on iMΦ. (C) Secretion of human IL-6 upon stimulation with lipopolysaccharide (LPS). iMΦ from human iPSC lines CD34iPSC16exi (non-targeted), iCasp9-mono (clone #12) or iCasp9-bi (clone #1) were used. MEF cells served as a negative control and human PB-MΦ as a positive control (n = 2-3). n.d, not detected (<1.6 pg/mL); ns, not significant.