Figure 2.

Gene expression in osteotomy hematoma tissue at 1, 4, 12, 24, 36 and 48 h post osteotomy. Samples were generated in an earlier study and gene expression of selected targets of interest was analyzed. HIF-1α, GLUT1 and HMOX1 have been previously published (adapted from [25]). HIF-1α and HMOX1 expression was analyzed starting from 12 h post fracture. Results are depicted as scatter plots, each point represents the mean gene expression detected in the osteotomy hematoma for one animal. All samples were measured in triplicate. For all animals per time point (n = 6) the mean gene expression ± SD is shown. Different signs (circle, square, triangle and rhombus) indicate the different time points. ND: non-detectable expression.