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. 2020 Mar 25;21(7):2259. doi: 10.3390/ijms21072259

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Dose-dependent positive regulation of the PI3K/Akt/mTOR signaling pathway by copaiba essential oil. (A) Pan-Akt profiles and (B) expression levels of the mTOR and p70S6K phosphoisoforms as functions of copaiba essential oil dose. (C) Relative concentrations of the Akt1 (blue), Akt2 (orange), Akt3 (gray), mTOR (yellow), and p70S6K (purple) phosphoisoforms as functions of copaiba essential oil dose. The relative concentration describes the fold change in a protein phosphoisoform after treatment compared to the control condition. The error bars are the standard deviations of six repeated measurements per experimental condition. SH-SY5Y cells were treated with various concentrations of copaiba essential oil for 30 min.