Skip to main content
. 2020 Apr 10;21(7):2646. doi: 10.3390/ijms21072646

Figure 1.

Figure 1

Blocking CD44 inhibits osteoclast differentiation of IgSF11-deficient cells in high-cell-density cultures. High-cell-density cultures of IgSF11+/+ and IgSF11−/− BMMs were treated with the indicated doses of soluble antibodies (A) anti-E-cadherin, (B) anti-CD9, (C) anti-CD44, and (D) anti-CD47, and cultured with M-CSF + RANKL for three days to induce osteoclast differentiation. On day three, cells were fixed and stained with TRAP (left). TRAP+ multinucleated cells (three nuclei or more per cell) were counted and the frequency of TRAP+ multinucleated cells is shown (right). Scale bars represent 100 μm. Data are shown as the mean ± S.D. *** p < 0.001, **** p < 0.0001.