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. 2020 Apr 8;21(7):2591. doi: 10.3390/ijms21072591

Figure 2.

Figure 2

Cytotoxic effect of ICMD-01 (A) and its modulation of nitric oxide production on macrophages (B). Panel (A): J774 cells were incubated with vehicle (5% DMSO in saline, control group) or different concentrations of ICMD-01 (ICMD-01; 6.25, 12.5, 25, 50 or 100 μM) for 72 h, and cell viability was determined by Alamar Blue assay. Gentian violet (GV) was used as the positive control. Data are expressed as means ± SEMs; n = 4 determinations per group. * Significantly different from the vehicle treated cultures (p < 0.05). ANOVA followed by Tukey’s multiple comparison test. Panel (B): Concentrations of nitrite were determined in J774 macrophages treated with vehicle (5% DMSO in saline, control group), IMCD-01 (ICMD-01; 6.25, 12.5, 25, 50 or 100 μM) or dexamethasone (Dexa; 40 μM) in the presence of LPS (500 ng/mL) plus IFN-γ (5 ng/mL). Cell-free supernatants were collected 24 h after treatments for nitrite quantification by the Griess method. Ct- shows concentrations of nitrite in unstimulated cells. Data are expressed as means ± SEMs; n = 6 determinations per group. * Significantly different from the vehicle treated cultures stimulated with LPS + IFN-γ (p < 0.05). ANOVA followed by Tukey´s multiple comparison test.