Figure 3.

The effect of ICMD-01 on cytokine production by activated macrophages. Concentrations of TNF-α and IL-1β were determined in cultures of J774 macrophages (Panels (A) and (B)) treated with vehicle (5% DMSO in saline, control group), IMCD-01 (ICMD-01; 6.25, 12.5, 25, 50 or 100 μM) or dexamethasone (Dexa; 40 μM) in the presence of LPS (500 ng/mL) plus IFN-γ (5 ng/mL). Cell-free supernatants were collected 4 h (for TNF-α measurement) and 24 h (for IL-1β) after treatments for ELISA assays. Ct—shows cytokine concentrations in unstimulated cell. Data are expressed as means ± SEMs; n = 4 for TNF-α and n = 6 IL-1β determinations per group. * Significantly different from the vehicle treated cultures stimulated with LPS + IFN-γ (p < 0.05). ANOVA followed by Tukey´s multiple comparison test.