Figure 6.
The effect of ICMD-01 on cytokine paw levels during CFA-induced inflammation. Mice were injected with ICMD-01 (50 mg kg−1), vehicle p.o. (5% DMSO in saline; control group) orally, or dexamethasone (intraperitoneal route) (Dexa; 2 mg kg−1; reference drug) route 40 min before CFA (injected at time zero). The naïve group consisted of mice that did not receive any experimental manipulation. Panels shows the paw levels of (A) interleukin-1β (IL-1β); (B) tumor necrosis factor-α (TNF-α), determined in skin tissues samples by ELISA, 3 h after the CFA injection. The results are expressed as picograms of cytokine per milligram of protein. Data are expressed as means ± SEMs; n = 6 mice per group. * Significantly different from the vehicle group in the same time (p < 0.05); # significantly different from the naive group (p < 0.05). ANOVA followed by Tukey´s multiple comparison test.