Skip to main content
. 2020 Mar 26;21(7):2302. doi: 10.3390/ijms21072302

Figure 3.

Figure 3

Evaluation of strand breaks (SBs) by X-ray irradiation and enhancement of SBs by PpIX for plasmid pBR322. (A) Agarose gel electrophoresis (0.7% agarose) of plasmids irradiated with X-ray at different concentrations of PpIX. “M” is the DNA ladder [OneSTEP Marker 6 (λ/Sty I digest), Nippon Gene, Japan]. (B) The fluorescence intensity of (A) was quantified, and the ratio of supercoiled to relaxed plasmid was calculated. (C) Capillary gel electrophoresis of DNA ladder mixed with PpIX and irradiated with X-ray. The 50 bp and 10,380 bp peaks belong to an internal standard marker.