Figure 2.

Transcriptional activity of NF-κB-BSs assigned to CASP9. (A) Schematic showing the luciferase constructs prepared with the pGL4.10 basic vector (pGL). mpGL, pGL with a minimal promoter sequence; BS10-mpGL, mpGL with a 218-bp sequence of BS10; mBS10-mpGL, BS10-mpGL with a mutated κB site. (B,C) Relative transcriptional activity in HeLa cells determined through a dual-luciferase reporter assay. C, control cells; T, TNFα-treated cells; S, RelA siRNA and TNFα co-treated cells. The data are presented as the means ± SEM from three independent experiments with three replicates in each test. The significance of the difference was analyzed by one-way ANOVA. * p < 0.05; ** p < 0.01.