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. 2020 Mar 26;21(7):2290. doi: 10.3390/ijms21072290

Figure 5.

Figure 5

MiR1276-specific binding to the 3ʹUTR of CASP9. (A) Binding site in 3′UTR of the CASP9 mRNA predicted by the TargetScan online program. (B) Relative luciferase activity analyzed by the dual-luciferase reporter assay in HeLa cells. The psiCHECK2-CASP9 3′UTR (3′UTR) or psiCHECK2-CASP9 3′UTR mutant (3ʹUTR mut) was co-transfected into HeLa cells with miR1276 expression plasmid (miR1276) or its negative control plasmid (NC). Results are presented as means ± SEM from three independent experiments with at least three replicates in each test. 3′UTR+NC vs. 3′UTR+miR1276 were analyzed with the Student’s t-test. * p < 0.05.