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. 2020 Mar 26;21(7):2290. doi: 10.3390/ijms21072290

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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TNFα promotion of apoptosis induced by DOX. (A) Detection of the cell viability by CCK8 assay. (B) Detection of cell apoptosis by flow cytometry (FCM) assay. The sub-G1 peaks of FCM assay were labeled with red arrows. The average apoptotic rates of three FCM detections were shown by histogram. The data are presented as the means ±SD from three independent experiments. C, control cells; T, TNFα-treated cells; T+D, TNFα&DOX-treated cells; D, DOX-treated cells. The significance of the difference was analyzed by one-way ANOVA. * p < 0.05; ** p < 0.01.