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Src activity is partially responsible for the dysregulation of PLEKHA7 and RNAi components in colon cancer cells. HT-29, DLD-1, and LS174T colon cancer cells were treated with the Src inhibitor PP2 or DMSO vehicle (control) for 24 h and: (A–C) stained by immunofluorescence for PLEKHA7, p120, DROSHA, Ago2 and imaged by confocal microscopy (scale bars: 20 μM); (D,E) subjected to western blot for the markers shown. Actin is the loading control.
