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. 2020 Mar 30;21(7):2390. doi: 10.3390/ijms21072390

Figure 6.

Figure 6

Responses in the right somatosensory cortex to electrical stimulation applied to the left forepaw and hindpaw in the absence (-) or presence (+) of SCS in the SNI model. An example of excitatory propagation (a) and the trace of optical signals (b) during stimulation of forepaw in a rat without SCS. In (a), the amplitude of the optical signal (ΔF/F) is color-coded, and the time from the onset of the stimulation of the hindpaw is shown at the top of each panel. The trace in (b) is obtained in the region of interest (ROI) indicated by the white circle in (a). Vertical lines in (b) indicate the applied 5 voltage stimuli. C, caudal; L, lateral; M, medial; R, rostral. An example of excitatory propagation (c) and the trace of optical signals (d) during stimulation of the hind paw in a rat without SCS is shown. An example of excitatory propagation (e) and the trace of optical signals (f) during stimulation of the hind paw in a rat with SCS is shown. (g) The initial responses (circles) and activated areas (colored areas) in forepaw stimulation (green) and hind paw stimulation (magenta) without SCS are shown. Note that the region responding to hind paw stimulation was medial and caudal to the region responding to forepaw stimulation. The data shown in (a–d), and (g) were obtained from the same animal. (h). Summary of peak amplitude, duration above 7SD, and the sum of amplitude exceeding 7SD obtained in the initial responses. Note that there are no significant differences between the control group (n = 6) and SCS group (n = 6). (i). Initial responses (circle) are aligned and activated areas in the control and SCS groups are superimposed. The number of overlapping responses is represented by the density of color. Magenta outlines indicate the overlapping areas in four of six rats. In the right panel, the outlines were merged. Solid and dashed lines indicate control and SCS groups, respectively. (j). Comparison of activated areas in response to hind paw stimulation without and with SCS. *p < 0.05. (Student’s t-test).