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. 2020 Mar 31;21(7):2434. doi: 10.3390/ijms21072434

Figure 2.

Figure 2

(A) RT-PCR analysis of exon-23 skipping induced by antisense oligonucleotides (AO) NAC 9078 and the corresponding 2′-OMePS control AO in H2K mdx mouse myotubes (gel image A corresponds to one of the three gels used for data in (B)); (B) densitometry analysis of exon-23 skipping induced by AO NAC 9078 and the corresponding 2′-OMePS control AO (triplicates) in H2K mdx mouse myotubes. Concentration range 2.5–100 nM. Grey, full-length product exon 20–26; green, exon-23 skipped product; blue, dual exon 22/23 skipped product; UT, untreated; NC, negative control (Error bars represent the standard deviation of mean).