Figure 2.

HEK cell electrophysiology voltage protocols. Cells were held at −100 mV for all three protocols. (A) Voltage protocol for the analysis of inactivation and transient currents. The 500 ms depolarizing prepulses from −130 to +10 mV, in 10 mV steps, followed by a 20 ms test pulse at +10 mV. (B) Resurgent current protocol. (C) Voltage protocol for the analysis of activation, transient currents, persistent currents, and gating pore currents. The 50 ms square pulses from −80 to +60 mV, in 5 mV steps.