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. 2020 Apr 1;21(7):2454. doi: 10.3390/ijms21072454

Figure 4.

Figure 4

Effects of cannabidiol (CBD) on wild-type (WT) hNav1.2 in stable human embryonic kidney (HEK) cell lines. The Navβ4 peptide was included in the pipette solution for all experiments with CBD. Unless otherwise indicated, * p < 0.05, *** p < 0.001. (A) The maximal peak transient current density of each cell is plotted; average and SEM for each group is indicated by bars. Significance was determined by Kruskal–Wallis test. n = 9 untreated, 7 vehicle (methanol), 8 CBD. (B) The maximal peak resurgent current density of each cell is plotted; average and standard error of the mean (SEM) for each group is indicated by bars. Significance was determined by two-way ANOVA with Tukey’s multiple comparisons. *p < 0.05, CBD vs. vehicle. n = 9 untreated, 7 vehicle (methanol), 8 CBD. (C) The maximal peak persistent current density of each cell is plotted; average and SEM for each group is indicated by bars. Significance was determined by Kruskal–Wallis test with Dunn’s multiple comparisons. n = 9 untreated, 7 vehicle (methanol), 8 CBD. (D) Average current densities elicited by voltage steps from −80 to +10 mV. Significance was determined by two-way ANOVA with Tukey’s multiple comparisons. *p < 0.05, CBD vs. vehicle, #p < 0.05, CBD vs. untreated, ##p < 0.05, vehicle vs. untreated. n = 9 untreated, 7 vehicle (methanol), 8 CBD. (E) Average peak resurgent current densities over a range of voltages from −65 to +5 mV. Significance was determined by Kruskal–Wallis test. n = 9 untreated, 7 vehicle (methanol), 8 CBD.