Expression of phospho c-Jun in kidney and spleen cells. Representative photograph of phospho c-Jun expression in the kidney after lipopolysaccharide (LPS) injection (A,B). In saline-treated control mice, phospho c-Jun-positive tubules were present in the kidney (A). Fewer c-Jun-positive cells were seen in rTM-treated mice than in the control mice (B). The number of c-Jun-positive tubules in 10 × 400 fields (C). Activation of splenic phospho c-Jun were assayed as protein levels by Western blotting (D,E). The data are the mean ±standard error of the mean (* p < 0.05 and ** p < 0.01, cell numbers and band density in control versus rTM-treated mice). Scale bar, 5 μm.