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. 2020 Mar 27;21(7):2332. doi: 10.3390/ijms21072332

Figure 3.

Figure 3

(A) Relative changes in transcript levels of leptin in mature Atlantic salmon adipocytes incubated with oleic, palmitic, or eicosapentaenoic acid for 72 h (Control) and serum-deprived in the presence of glucagon thereafter for 18 h (18h GLU). Data are shown as mean ± SEM (n = 4). Results are compared by two-way ANOVA using the fatty acid tested (OA, PA, and EPA) and the experimental condition (Control and 18h GLU) as factors. (B) Changes in intracellular leptin concentrations in mature Atlantic salmon adipocytes incubated with oleic, palmitic, or eicosapentaenoic acid for 72 h (Control) and serum-deprived in the presence of glucagon thereafter for 3 (3h GLU) and 18 h (18h GLU). Data are shown as mean ± SEM (n = 3). Results are compared by two-way ANOVA using the fatty acid tested (OA, PA, and EPA) and the experimental condition (Control, 3h GLU, and 18h GLU) as factors. Lowercase letters indicate significant differences between fatty acids, and capital letters indicate significant differences between conditions (p < 0.05; Tukey’s post hoc test). Relative changes in leptin transcript levels were analyzed with real-time qPCR; data are presented as −ΔΔCt ± SEM, and the OA group from the Control condition was set to zero. OA = oleic acid, PA = palmitic acid, and EPA = eicosapentaenoic acid.