Figure 6.

Total glycerol (A), non-esterified free fatty acids (NEFA) (B), and [1-¹⁴C] from radiolabelled PA recovered in secreted lipids (C) in the media from mature Atlantic salmon adipocytes incubated with oleic, palmitic, or eicosapentaenoic acid for 72 h and serum-deprived in the presence of glucagon thereafter for 3 (3 h GLU) and 18 h (18 h GLU). Data are shown as mean ± SEM (n = 4 for glycerol and NEFA data, and n = 3 for radiolabeled lipids secreted data). Results are compared by two-way ANOVA using the fatty acid tested (OA, PA, and EPA) and the experimental condition (3 h GLU and 18 h GLU) as factors. Lowercase letters indicate significant differences between fatty acids (p < 0.05; Tukey’s post hoc test). Asterisks (*) indicate significant differences between conditions (p < 0.05; Sidak’s test). OA = oleic acid, PA = palmitic acid, and EPA = eicosapentaenoic acid.