Table 2.
Relative distribution of radioactivity from [1-14C PA] recovered in different cellular lipid classes in Atlantic salmon differentiated adipocytes incubated with oleic (OA), palmitic (PA), or eicosapentaenoic acid (EPA) for 72 h.
| (%) | OA | PA | EPA | p |
|---|---|---|---|---|
| PL | 46.17 ± 3.37 b | 47.26 ± 7.02 b | 82.33 ± 2.71 a | 0.0026 |
| FFA | 2.63 ± 0.25 b | 12.00 ± 2.28 a | 3.35 ± 0.97 b | 0.0064 |
| TAG | 5.84 ± 1.10 b | 11.28 ± 1.32 ab | 11.93 ± 1.55 a | 0.0338 |
| DAG | 2.08 ± 0.43 | 1.9 ± 0.72 | 2.39 ± 0.67 | 0.8583 |
| CE | 43.29 ± 4.67 a | 27.56 ± 4.56 b | ND | 0.0367 |
| Total nmol 1 | 5.8 ± 0.35 a | 1.8 ± 0.07 b | 6.7 ± 0.46 a | 0.0001 |
Data are shown as mean ± SEM (n = 3). Different letters indicate significant differences between the experimental groups (p < 0.05; one-way ANOVA followed by Tukey’s honestly significant difference test). The recovery values for the OA, PA, and EPA group were 87.27 ± 2.91%, 97.35 ± 3.05%, and 89.51 ± 3.18% of the initial radiolabelled PA, respectively. ND, not detectable levels. 1Total nmol: total nmol radiolabeled lipids recovered in the cellular lipid fraction. PL = phospholipids, FFA = free fatty acids, TAG = triglycerides, DAG = diacylglycerol, and CE = cholesteryl esters.