Figure 2.

Analysis of SaOS2 cells cultured in 3D collagen scaffolds (3 and 7 days) treated with the osteogenic cocktail (20 nM 1α,25-Dihydroxyvitamin D3, 50 µM L-Ascorbic acid 2-phosphate, 10 mM β-Glycerophosphate). qPCR analysis of osteogenic markers (RUNX2, COL1A1, BGLAP, and SPP1) was performed using GAPDH and HPRT1 as reference genes (2-ΔΔCT method). Fold changes from control untreated cells at day 3 were calculated. Data are reported as mean ± SEM of three biological replicates. * p < 0.05; ** p < 0.01.