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. 2020 Apr 6;21(7):2545. doi: 10.3390/ijms21072545

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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pCMV-BAM-hDENND1A.V2 expression construct. (A) Map for pCMV-BAM-hDENND1A.V2 construct. (B) Efficiency of the vector was tested by examining hDENND1A.V2 expression by Western blot and immunodetection in transfected COS-I and MA-10 cells. pCMV-BAM was used as the empty construct (negative control). (C) Immunofluorescence detection of hDENND1A.V2 in CHO cells transfected with the pCMV-BAM-hDENND1A.V2 construct. (D) Representative PCR results for the genotyping of wild-type and transgenic mice generated using the pCMV-BAM-hDENND1A.V2 vector. WT, wild-type; Tg, transgenic; B, blank; V2.CMV, pCMV-BAM-hDENND1A.V2 vector.