Figure 7.

Conversion of HPG into active plasmin by HRgpA and Kgp in the presence of PPAD. A total of 300 nM HPG was incubated with 3 nM HRgpA and Kgp and 100 nM PPAD for 2 h at 37 °C. The gingipain inhibitors KYT-1 and KYT-36 were then added at a final concentration of 1 µM and the resulting plasmin activity in the presence of plasmin substrate was followed by monitoring the absorption of the released p-nitroaniline at 405 nm. Bars represent the mean values ± SEM. Untreated HPG served as a control. Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparisons test (p < 0.05) using GraphPad Prism. Statistical significance levels are indicated: * p< 0.05; ns, non-significant; # p < 0.05 vs. control.