Figure 1.

Versican fragments containing the neo-epitope DPEAAE are detected in OCCs expanded in vivo. The complexes expanded in vivo were isolated from naturally cycling gilts (two porcine OCCs for each lane). Total (T), matrix (M) and cell (C) extracts from expanded OCCs digested with Streptomyces hyaluronidase (H) or chondroitinase ABC (CH) were analyzed by western blot with anti-DPEAAE versican antibody. Actin immunodetection was performed to compare sample loading. A representative blot of four independent experiments is shown.