Figure 3.

SLP-2KO Inhibits Cell Migration and Invasion in CRC Cells

(A) Representative images of the migration of HCT116^SLP-2KO, HCT116^EV, SW480^SLP-2KO, and SW480^EV cells, as analyzed using a wound-healing assay at 0 and 24 h. Magnification: 100×. (B) The bar plots indicate the wounded area of each cell line. (C) Representative images of the invasion of HCT116^SLP-2KO, HCT116^EV, SW480^SLP-2KO, and SW480^EV cells, as analyzed using a Transwell assay at 12 h after seeding. Magnification: 100×. (D) The bar plots indicate the number of invaded cells of each cell line. Error bars in (B) and (D) indicate mean ± SEM (n = 3). ∗∗∗p < 0.001, two-tailed, unpaired t tests. (E) Representative western blot analysis of MMP2 in HCT116^SLP-2KO, HCT116^EV, SW480^SLP-2KO, and SW480^EV cells. GAPDH was used as a protein loading control.