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. 2020 Mar 25;21(7):2260. doi: 10.3390/ijms21072260

Figure 6.

Figure 6

ALA ameliorates radiation-induced impairment of stem cells. Sprague–Dawley rats were subjected to 18 Gy radiation in the head and neck region. (A and C) SGs were collected at each time points, and RNA was extracted and subjected to reverse-transcription PCR analysis of Sca-1. GAPDH was used as the loading control. The expression level of Sca-1 is normalized to GAPDH. The expression in each group was represented as fold change. Con; control (n = 3). ALA; ALA only treated group (n = 3). RT; irradiation only group (n = 3). ALA+RT; ALA plus irradiation groups (n = 3). (B and D) The sections were immunostained with anti-c-Kit. Figures are representative images from each group. c-Kit positive signals on SG sections (B) and the quantification of staining intensity (D). Scale bar, 50 μm. N = 3–4 SGs/group. The fold change is calculated as the ratio of the final value in each group to the value in control group at 2 weeks (set as “1”). Values are represented as the mean ± SEM. * p < 0.05.