Figure 5.

let-7 Mimics Block the Tumor-Promoting Effects of circHMCU Overexpression by Downregulating HMGA2, MYC, and CCND1

(A) Transwell assays of cell migration after transfection with circHMCU and let-7 mimics in MDA-MB-231, MDA-MB-468, and MCF-7 cells. (B) Wound healing assays after transfection with circHMCU and let-7 mimics in MDA-MB-231 cells. (C) Observation of DNA synthesis of MCF-7 cells transfected with circHMCU overexpression vectors and let-7 mimics by EdU assay. (D) MTT analysis of cell proliferation after transfection with circHMCU overexpression vectors and let-7 mimics in MDA-MB-231 and MCF-7 cells. (E) Kaplan-Meier survival curve shows the relationship between HMGA2/CCND1/MYC expression and survival rates of breast cancer patients. (F) Western blot analysis of the expression levels of HMGA2, MYC, and CCND1 after transfection with circHMCU and let-7 in MDA-MB-231 cells. Data are the means ± SEM of three experiments. ∗p < 0.05, ∗∗p < 0.01