Figure 6.

circHMCU Promoted Breast Cancer Cell Growth and Metastasis in a Xenograft Model

(A) Photograph illustrated tumors in xenografts. Overexpression of circHMCU promoted the growth of tumors. (B) Expression of circHMCU in the xenograft model. (C) Growth curve of xenograft tumors after tumor cells injection. (D) Overexpression of circHMCU promoted lung metastasis of MDA-MB-231 cells. (E) Representative images of hematoxylin and eosin (H&E) staining of lungs isolated from mice that received tail vein injection of MDA-MB-231 pLCDH-ciR (empty vector) cells and circHMCU overexpression cells. Scale bars, 200 μm. (F) IHC staining analysis revealed the increased expression of proliferation index Ki-67 and HMGA2/MYC/CCND1 expression in circHMCU overexpressed tumors compared with control group. Scale bars, 100 μm. (G) IHC staining analysis of the expression of HMGA2/MYC/CCND1 in the tissues of breast cancer patients with different circHMCU expression levels. Scale bars, 100 μm. Data are the means ± SEM of three experiments. ∗∗∗p < 0.001.