Table 1.
Sequences of primers used for qPCR.
| Gene group | Gene | Accession no. | Sequence | Product Size (bp) |
|---|---|---|---|---|
| Ligands regulating T cell activation | PD-L2 | XM_847012.5 | F: ACTGGCTGCTTCATGTTGTC | 169 |
| R: CAGGTTCAAATAGCTCCGTCC | ||||
| Gal9 | NM_001003345.1 | F: GCTGCGATTTCAAGGTGACG | 134 | |
| R: GCCTGGAGACTGGAAGCTAA | ||||
| CD8 | NM_001003146.2 | F: GAAACCCACCCCTGATGGAG | 191 | |
| R: ACCGTACTCTTTCCTTGGTCTG | ||||
| T cytotoxic cell-associated genes | CD8α | NM_001002935.2 | F: GTGGGTTAGACTTCGCCTGT | 117 |
| R: CACGTCTTCTGTTCCTGTGGT | ||||
| IFN-γ | AF126247.1 | F: TCAAGGAAGACATGCTTGGCAAGTT | 74 | |
| R: GACCTGCAGATCGTTCACAGGAAT | ||||
| Th1 cell-associated genes | CXCR3 | NM 001011887.1 | F: TGGATGTGGCCAAGTCTGTC | 200 |
| R: TGAGGGGGTCTCGGACCAG | ||||
| TBX21 | XM_548164.4 | F: AAGCAGGGGCGGCGGATGTT | 139 | |
| R: ACTGCACCCACTTGCCGCTC | ||||
| Th2 cell-associated genes | IL-4 | AF187322.1 | F: TCACCAGCACCTTTGTCCACGG | 96 |
| R: TGCACGAGTCGTTTCTCGCTGT | ||||
| GATA3 | XM 844060.1 | F: CGAAGGCTGTCGGCAGCAAGAA | 98 | |
| R: ACGGGGTCTCCGTTGGCATT | ||||
| CCR2 | XM 541906.1 | F: ACATGCTGTCCACATCGCA | 91 | |
| R: GGCGCGCTGTAATCATAGTC | ||||
| Anti-inflammatory and pro-inflammatory cytokines | IL-10 | NM 0010030771 | F: CAGGTGAAGAGCGCATTTAGT | 107 |
| R: TCAAACTCACTCATGGCTTTGT | ||||
| IL-12A p35 | NM 001003293.1 | F: TGCCTGGCCTCTGGAAAG | 74 | |
| R: TACATCTTCAAGTCCTCAT | ||||
| IL-12B p40 | NM 001003292.1 | F: GCCAAGGTCGTGTGCCA | 81 | |
| R: CCAGTCGCTCCAGGATGAAC | ||||
| Th17 cell-associated genes | IL-17 | NM 001165878.1 | F: CACTCCTTCCGGCTAGAGAA | 71 |
| R: CACATGGCGAACAATAGGG | ||||
| RORγt | XM 540323.3 | F: TCAACGCCAACCGTCCGGG | 143 | |
| R: CCGAAGCTTCCCCTTGGGCG | ||||
| Housekeeping genes | HPRT | NG_042858.1 | F: TTATAGTCAAGGGCATATCC | 104 |
| R: AGCTTGCTGGTGAAAAGGAC | ||||
| RPS19 | XM_005616513.3 | F: GTTCTCATCGTAGGGAGCAAG | 95 | |
| R: CCTTCCTCAAAAAGTCTGGG |