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. Author manuscript; available in PMC: 2020 Oct 20.
Published in final edited form as: Immunohorizons. 2019 Nov 20;3(11):547–558. doi: 10.4049/immunohorizons.1800075

FIGURE 6. IL-12–induced p-STAT4 inhibition is intact in T24D, A50H mutant SOCS3–transduced cells.

FIGURE 6.

(A) Schematic showing experimental design; (B and C) T cells containing empty vector, WT SOCS3, or SOCS3-DH were sorted for CD8+clover+ cells, treated with IL-12 or no cytokine, then lysed and protein subjected to Western blotting to detect p-STAT4 (B) or total STAT4 (C). Whole spleen cells served as a positive control for IL-12–induced p-STAT4. Pervanadate is a protein tyrosine phosphatase inhibitor serving as chemical method to induce STAT4 phosphorylation. Representative data from two to three experiments.