Figure 6.

Effect of OLE and HT on rotenone-induced locomotion deficits. The administration of 10 µM rotenone, starting at the fourth larval stage, led to Parkinsonian-like phenotype exhibiting movement impairments at the 3^rd and 7^th day of adulthood. (A) The thrashing rate, (B) the body wave number, and (C) the activity index are shown with and without simultaneous OLE or HT administration. The number of tested nematodes was: DMSO control = 58 (A3) and 69 (A7); OLE = 60 (A3) and 63 (A7); water control = 62 (A3) and 46 (A7); HT = 44 (A3) and 61 (A7). A3, A7: day 3 and 7 of adulthood. Data are pooled from two biological repeats and presented as mean ± SEM, and differences compared to control were considered significant at p < 0.05 (*) and p < 0.001 (**). To enable direct comparisons, data from nematodes without rotenone and polyphenol exposures (see Figure 5) are shown in addition.