Figure 4.

Reactive oxygen species (ROS) production and morphological changes. (A) Effect of compounds 1–8 on hydrogen peroxide formation in BxPC3 cells. BxPC3 cells were pre-incubated in phosphate-buffered saline (PBS)/10 mM glucose medium for 20 min at 37 °C in presence of 10 µM 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate acetyl ester (CM-DCFDA) and then treated with the IC₅₀ of copper(I) compounds as a function of time (minutes). (B) TEM analysis. Transmission electron micrographs of BxPC3 cells after 24 h or 48 h of treatment with complex 8: a and b, controls; c, 24 h compound 8; d, 48 h compound 8.