Figure 2. The regulatory effects of DRG1 on various hallmarks of OS cells.

To knock down DRG1, the present study designed three siRNAs to target DRG1. siRNA3 showed the highest efficiency in DRG1 knockdown, thus it was used in further study (A). After DRG1 knockdown, cell viability (B), apoptosis rate (C), cell cycle distribution (D), expression of Cycline B and CDK1 (E), cell migration (F), invasion (G), and colony formation rate (H) were evaluated. Silencing of Drg1 resulted in decreased viability and inhibition of the migration and colony formation abilities of OS cells; it also resulted in cell cycle arrest in the G₂/M stage and induced apoptosis. *P<0.05, **P<0.01, and ***P<0.001 vs. control.