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. 2020 Apr 21;40(4):BSR20200282. doi: 10.1042/BSR20200282

Figure 4. DRG1 expression was affected by METTL3 and ELAVL1 in m6A-dependent manner in OS cells.

Figure 4

As indicated by M6A-IP-qPCR assay, m6A level of DRG1 mRNA was higher in MG63 and 143B cells than in hFOB1.19 cells (A). **P<0.01 vs. hFOB1.19 cells. An m6A prediction website (http://m6avar.renlab.org/) showed an m6A position at 3′UTR of DRG1 mRNA (B) and proteins that showed high affinity to DRG1 mRNA (C). Among the proteins, ELAVL1 and IGF2BP2 are m6A readers. Bioinformatics analysis (http://gepia.cancer-pku.cn/) further showed that DRG1 expression was positively correlated to METTL3 and ELAVL1 expression, but not to IGF2BP2 expression (D). Silencing METTL3 and ELAVL1 significantly decreased both mRNA (E) and protein levels of DRG1 (F) in MG63 and 143B cells. Depletion of IGF2BP2 only decreased DRG1 mRNA and protein levels in 143B cells. m6A level of DRG1 mRNA was undermined after METTL3 knockdown (G). *P<0.05 and **P<0.01 vs. control. The stability of DRG1 mRNA was impaired after both METTL3 and ELAVL1 knockdown (H). *P<0.05 and **P<0.01 vs. 0 h. Abbreviation: KD, knockdown.