Figure 4.

Multiplexed measurements of three miRNA targets from total RNA derived from tissue. (A) The signal-to-noise ratio (SNR) of three miRNAs in tumor and healthy samples were plotted, and each of the tumor and healthy samples was measured 10 times. Error bars represent the standard deviation of targets normalized by assay noise (n = 10). (B) Dysregulation ratios of three miRNA targets in lung tumor versus healthy tissue were as expected from the previous studies, validating our assay multiplexing scheme. Error bars represent the standard deviation of miRNA expression measurements in tumor normalized by background-subtracted average miRNA signal in normal and by the ratio of tumor to normal miRNA expression.