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PCR amplification of tetracycline resistance genes using the universal primers Tet1 and Tet2 targeting a segment of 1,300 bp of the genes encoding RPP (A) and 1,200 bp of the tet(W) gene with the specific primer pair tetWF-Tet2 (B). Key of samples: Lane 1, DNA from B. bifidum VA07-1AN; lane 2, B. bifidum VA07-2AN; lane 3, Leuconostoc mesenteroides subsp. mesenteroides LbE16 (positive control) [9]; line 4, blank (no template DNA). M, molecular weight marker.
