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. 2020 Apr 8;21(7):2594. doi: 10.3390/ijms21072594

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Chromatograms of amplicons of the tet(W) gene from the original tetracycline-susceptible strain B. bifidum VA07-1AN and two representative tetracycline-resistant revertants (R-1 and R-11). Nucleotide sequences and the corresponding deduced amino acid sequences are displayed below each of the chromatograms. DNA and protein differences with canonical sequences of the tet(W) gene from the tetracycline-resistant Bifidobacterium longum LTBL16 strain (on top of the figure) are highlighted in red.