Figure 3.

Transcription of European sea bass IL-17 ligands is induced in head-kidney leucocytes by T cell mitogens. The relative gene expression of IL-17 ligands in the European sea bass head-kidney leucocytes incubated for 4 h with culture medium (Control), 5 μg/mL concanavalin A (ConA), 5 μg/mL lipopolysaccharide (LPS), 10 μg/mL phytohemagglutinin (PHA), 50 μg/mL synthetic unmethylated cytosine-phosphodiester-guanosine oligodeoxynucleotide 1668 (ODN), 25 μg/mL PolyI:C (pI:C), 10⁸ heat-killed Vibrio anguillarum (Va) or Photobacterium damselae (Pd) bacteria/mL, and 10⁶ TCID₅₀ nodavirus (NNV)/mL. The data are presented as the mean (n = 5) ± SEM relative to the expression of the endogenous controls. Different letters indicate differences in gene expression (ANOVA, p < 0.05).