Figure 1.

Murine B16-F10 Melanoma Cells Are Highly Permissive for IAV Infection

(A) B16-F10 cells were infected with different low pathogenic human (left panel) or highly pathogenic avian IAV strains (right panel) and virus titers in cell supernatants were determined by standard plaque assay at 24 h post-infection (hpi). B16-F10 cells were infected with an MOI of 0.01 with low pathogenic human IAVs (strains PR8, WSN, and Victoria) or a MOI of 0.001 with highly pathogenic avian IAVs (strains FPV and KAN-1). Virus titers are presented as plaque-forming units (PFU) per mL. (B) B16-F10 cells were infected with PR8 of different MOIs, and virus titers represented as PFU/mL were investigated at indicated time points post-infection by standard plaque assay. (C) mRNA expression levels of Fas and Tnfsf10 as marker genes for apoptosis induction were evaluated by qRT-PCR at different times of IAV infection. (D) Induction of cell death upon PR8 infection was investigated by fixable viability dye eFluor 450 staining and subsequent flow cytometry analysis at different time points post-infection. Mean values of three independent experiments ± SEM are shown.