Table 2.
Antibodies Used for Low Cytometry Analysis
| Marker | Fluorophore | Clone | Manufacturer |
|---|---|---|---|
| B7-H3 | APC | MIH35 | BioLegend |
| B7-H4 | PE | HMH4-5G1 | BioLegend |
| CD11b | Pacific Blue | M1/70 | BioLegend |
| CD11c | PE/Dazzle 594 | N418 | BioLegend |
| CD16/CD32 | – | 2.4G2 | BD Pharmingen |
| CD3 | PE/Cy7 | 145-2C11 | eBioscience |
| CD3 | PE/Dazzle 594 | 145-2C11 | BioLegend |
| CD4 | APC/Fire 750 | GK1.5 | BioLegend |
| CD49b | PE/Cy7 | DX5 | BioLegend |
| CD45.2 | Pacific Orange | 30-F11 | Invitrogen |
| CD68 | PerCP/Cy5.5 | FA-11 | BioLegend |
| CD8 | FITC | 53-6.7 | BioLegend |
| CD80 | PerCP/eFluor 710 | 16-10A1 | eBioscience |
| CD86 | APC | GL-1 | BioLegend |
| CTLA-4 | PE/Dazzle 594 | UC10-4B9 | BioLegend |
| F4/80 | PerCP/Cy5.5 | BM8 | BioLegend |
| FOXP3 | PE | FJK-16 s | eBioscience |
| IFN-γ | APC | XMG1.2 | BD Pharmingen |
| IL-4Rα | Alexa Fluor 647 | mIL4R-M1 | BD Pharmingen |
| Ly-6G | APC/Fire 750 | 1A8 | BioLegend |
| MHCII | PE/Cy7 | M5/114.15.2 | BioLegend |
| MHCII | APC/Fire 750 | M5/114.15.2 | BioLegend |
| NKp46 | PE | 29A1.4 | BioLegend |
| PD-1 | PE | 29F.1A12 | BioLegend |
| PD-1 | PerCP/Cy5.5 | 29F.1A12 | BioLegend |
| PD-L1 | PE | 10F.9G2 | BioLegend |
| PD-L2 | PE | TY25 | BioLegend |
| Siglec-F | PE | 1RNM44N | eBioscience |
APC, allophycocyanin; PE, phycoerythrin; PerCP, peridinin chlorophyll protein.