Figure 1.
Cytopathic Effects and Virus Yields of G47Δ in Human Gastric Cancer (GC) Cell Lines
(A) Cells were seeded onto six-well plates at 2 × 105 cells/well or onto 96-well plates at optimal cell density. After an overnight incubation, the cells were infected with G47Δ (44As3, HSC-39, MOI of 0.1 [○] or 1 [▲]; other cells, MOI of 0.01 [●], 0.1 [○], or mock). The cell viability was determined daily either by counting surviving cells with a Coulter Counter or by the CellTiter 96 AQueous non-radioactive cell proliferation assay. The percentage of surviving cells is expressed as a percentage of mock-infected controls on each day. G47Δ exhibited a good cytopathic effect in the majority of human GC cell lines tested at an MOI of 0.01, whereas some of the cell lines such as 44As3 and HSC-39 were resistant to G47Δ, even at an MOI of 0.1. Data are presented as the mean of triplicates ± SD. One-way ANOVA followed by a Dunnett’s test was used to determine statistical significance (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, NS, not significant; versus mock-infected controls). (B) Cells were seeded onto six-well plates at 3 × 105 cells/well. Triplicate wells were infected with G47Δ at an MOI of 0.01. At 24 or 48 h after infection, cells were collected and progeny virus was titered on Vero cells. In most cell lines tested, G47Δ showed good replication capability by 48 h after infection, but with exceptions such as 44As3. The results presented are the mean of triplicates ± SD.