Figure 2.

Antitumor Efficacy of G47Δ in GC Subcutaneous Tumor Models

(A) Subcutaneous tumors from three human GC cell lines (MKN45 [upper left], MKN74 [upper right], and 44As3 [lower left]) were generated in 6-week-old female athymic mice. Established tumors, 5–6 mm in diameter, were inoculated with G47Δ (2 × 10⁵ or 1 × 10⁶ PFU) or mock on days 0 and 3. G47Δ treatment significantly inhibited the tumor growth compared with mock treatment in all GC tumor models irrespective of the dose used. The results presented are the mean ± SEM (n = 7). (B) Established MKN45 and 44As3 subcutaneous tumors were inoculated with G47Δ (1 × 10⁶ PFU) or mock on days 0, 2, and 4 when the tumor volumes exceeded 200 mm³. G47Δ treatment significantly inhibited the tumor growth compared with mock treatment in both GC tumor models. The results presented are the mean ± SEM (n = 7). One-way ANOVA followed by a Dunnett’s test was used to determine statistical significance (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001; NS, not significant).