Figure 3.
Efficacy of G47Δ in an Orthotopic Scirrhous Gastric Tumor Model
(A) Experimental design. Female athymic mice were orthotopically inoculated with 44As3Luc cells (1.0 × 106 cells) into the gastric wall. After confirmation of the orthotopic tumors with IVIS imaging on day 7, the mice were divided into two groups and were treated by intratumoral inoculation with G47Δ (1 × 106 PFU) or mock on days 8 and 11. (B) The established orthotopic gastric tumor was macroscopically confirmed on day 8. (C) The photon counts of the tumors were calculated with IVIS imaging every 3–4 days. G47Δ treatment significantly decreased the total bioluminescence from tumors (p = 0.04 on day 27). (D) G47Δ treatment prolonged the survival of tumor-bearing mice (p = 0.004). (E) Two representative cases from each group are shown. The results presented are the mean ± SEM (n = 7). The Mann-Whitney U test was used to analyze the total bioluminescence from tumors, and the survival was analyzed by the Kaplan-Meier method using the log rank test (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001; NS, not significant).